A cell-permeable pyrrazolopyrimidine compound that inhibits against KDR/VEGFR2, ALK2/BMPR-I, AMPK kinase activity (IC
50 = 25.1, 148, and 234.6 nM, respectively), while exhibiting much reduced or little effect toward ALK5/TGFβR-I, ZAPK, Syk, PKCθ, PKA, or JAK3. Shown to block both BMP pathway-dependent dorsoventral development (EC
100 = 2.5 μM) and VEGF signaling-dependent intersomitic vessel formation (EC
50 = 5 μM) in zebrafish embryo
in vivo. Commonly used in combination with AMPK activators AICAR (Cat. No.
123040) and/or Metformin (Cat. No.
317240) for studying AMPK-dependent cellular events
in vitro and physiological responses in animals
in vivo.