ANTI-IL-12抗体

Anti-IL-12 Antibody, Rabbit Polyclonal Rabbit Reacts with: Human Recombinant Human IL12A & IL12B protein Produced in rabbits immunized with purified, recombinant Human IL12A & IL12B.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB2 antibody (ab203209)

Immunohistochemical analysis of formalin-fixed, paraffin embedded Human bladder carcinoma tissue labeling IL-12RB2 with ab203209 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Interleukin 12 (IL-12) family is unique in having the only heterodimeric cytokines, including IL-12, IL-23, IL-27 and IL-35. The heterodimeric cytokines of the IL-12 family consist of an α-chain (p19, p28 or p35) and a β-chain (p40 or Ebi3). The α-chains have a four-helix bundle structure characteristic of the IL-6 superfamily, to which the IL-12 family belongs. In contrast, the β-chains share homology with class I receptor chains for cytokines, such as IL-6Rα. The p40 chain can pair with p35 or p19 to form IL-12 or IL-23, respectively, whereas Ebi3 can pair with p28 or p35 to form IL-27 or IL-35, respectively. The receptor chains are also used by multiple cytokines. IL-12 signals via IL-12Rβ1 and IL-12Rβ2, whereas IL-23 signals via IL-12Rβ1 and IL-23R. In contrast, IL-27 uses gp130 and IL-27R (WSX-1),whereas IL-35 signals via gp130 and IL-12Rβ2.IL-35 is unusual in that it can also signal via two additional receptor-chain compositions: gp130-gp130 and IL-12Rβ2–lL-12Rβ2 homodimers. ANTI-IL-12抗体是一类可以特异性结合ANTI-IGE的多克隆抗体，主要用于检测ANTI-IGE的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。 白细胞介素-12是趋化因子家族的一种细胞因子，目前共发现了38个白细胞介素，分别命名为IL-1---IL38.功能复杂，成网络，复杂重叠。 白细胞介素(interleukin,IL)12,由抗原提呈细胞和B细胞产生，是一种异源二聚体形式的前炎症细胞因子，并以这种形式分泌到细胞外。IL-12能诱导IFN-γ产生，在体内免疫应答中（特别是在细菌或寄生虫感染中）它也是IFN-γ产生所必需。 来自活化淋巴细胞，由IL-12A(p35)和IL-12B(p40)亚基组成，二者分别与IL-6Rα链和IL-6同源。是NK细胞激活因子，能促进CD4+Th0细胞分化为Th1细胞，协同亚剂量IL-2，可诱导LAK细胞活性。 双抗体夹心法测定标本中ANTI-IGE水平。用纯化的ANTI-IGE抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入ANTI-IGE，再与HRP标记的ANTI-IGE抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的ANTI-IGE呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中ANTI-IGE浓度。