PAMAM dendrimer generation&

PAMAM dendrimer G2.5 carboxylate is a polyamidoamine (PAMAM) dendrimer with carboxylate termini (PAMAM G2.5).1 It is approximately 48.3 Å in diameter in water and has 24 surface groups.2 PAMAM G2.5 binds to cytochrome c and decreases its hydrolysis by chymotrypsin by approximately ~30% in a cell-free assay when used at a concentration of 1 µM. When coupled to polyethylenimine (PEI), PAMAM G2.5 co-assembles with DNA into dendrosome nanoparticles (DNPs), which increase transfection efficiency and decrease DNA nuclease degradation in HeLa cells.3 When coupled to the adenosine A1 receptor agonist adenosine amine congener (ADAC; Item No. 34394), PAMAM G2.5-ADAC inhibits forskolin-induced activation of adenylyl cyclase in CHO cells expressing the human adenosine A3 receptor (EC50 = 1.6 nM).4WARNING This product is not for human or veterinary use.

[1] FUMIKO CHIBA. Dendritic Macromolecules as Inhibitors to Protein-Protein Binding[J]. Macromolecular Symposia, 2010, 287 1: 37-41. DOI: 10.1002/masy.201050106
[2] GABRIELLA CAMINATI  Donald A T  Nicholas J Turro. Photophysical investigation of starburst dendrimers and their interactions with anionic and cationic surfactants[J]. Journal of the American Chemical Society, 1990, 112 23: 8515-8522. DOI: 10.1021/ja00179a041
[3] SHIRONG PAN. A Serum-Resistant Low-Generation Polyamidoamine with PEI 423 Outer Layer for Gene Delivery Vector[J]. Macromolecular bioscience, 2013, 13 4: 422-436. DOI: 10.1002/mabi.201200255
[4] ATHENA M KLUTZ. Enhanced A3 adenosine receptor selectivity of multivalent nucleoside-dendrimer conjugates.[J]. Journal of Nanobiotechnology, 2008, 6: 12. DOI: 10.1186/1477-3155-6-12