4-Nitrophenyl Palmitate is used in immobilization method of lipase interface based on natural polysaccharide particles.
ChEBI: A palmitate ester obtained by condensation of the carboxy group of palmitic acid with the phenolic hydroxy group of p-nitrophenol.
4-Nitrophenyl palmitate is a substrate for lipase enzyme activity. Lipase hydrolyzes 4-nitrophenyl palmitate and yields the yellow colored product 4-nitrophenol, which is measurable spectrophotometrically at 410 nm. This method is advantageous due to its short reaction time and facile spectrophotometric analyses. The cell-bound lipase has preference for 4-nitrophenyl palmitate as substrate than the extracellular lipase.
