MEOSUC-ALA-ALA-PRO-VAL-AMC

Fluorogenic substrate for quantification of human leukocyte and porcine pancreatic elastase

N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin has been used as a substrate in elastase assay using bone marrow lysates and pancreatic lysates.

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 μM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.

Km = 140 μM; kcat/Km = 120,000 M?1 s?1as substrates for human leukocyte and porcine pancreatic elastase