Uses
Fluorogenic substrate for quantification of human leukocyte and porcine pancreatic elastase
Uses
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin has been used as a substrate in elastase assay using bone marrow lysates and pancreatic lysates.
General Description
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K
m = 140 μM; K
cat/K
m = 120,000 s
-1 M
-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.
Biochem/physiol Actions
Km = 140 μM; kcat/Km = 120,000 M?1 s?1as substrates for human leukocyte and porcine pancreatic elastase
