里查酮 A
里查酮 A 性质
| 沸点 | 649.7±55.0 °C(Predicted) |
|---|---|
| 密度 | 1?+-.0.06 g/cm3(Predicted) |
| 储存条件 | 2-8°C |
| 溶解度 | 溶于氯仿、二氯甲烷、乙酸乙酯、DMSO、丙酮等。 |
| 形态 | 粉末 |
| 酸度系数(pKa) | 7.67±0.40(Predicted) |
| 颜色 | 米白色至浅黄色 |
里查酮 A 用途与合成方法
Leachianone A (0-20 μg/ml; 24-72 hours) exhibits a marked inhibition on the survival of HepG2 cells time- and dose-dependently manner, IC 50 values are 6.9 μg/ml, 3.4 μg/ml and 2.8 μg/ml in cells with 24-, 48- and 72-hours treatment, respectively. Leachianone A (10-30 μg/ml; 48 hours) indicates that at low concentration of LA (10 μg/ml), a substantial amount of cells is primarily in the early phase of apoptosis, at higher concentrations, induces a shift of the cell population to late apoptotic/ necrotic stage. Leachianone A (10-30 μg/ml; 48 hours) decreases the precursor of caspase-3 in a dose-dependent manner, reduces the protein level of the pro-forms of upstream initiator caspases, caspases-8 and -9, decreases two downstream substrates, namely inhibitor of caspase-activated DNase(ICAD) and poly-ADP-ribose polymerase (PARP) in HepG2 cells.
Cell Viability Assay
| Cell Line: | HepG2 cells |
| Concentration: | 0 μg/ml, 2 μg/ml, 4 μg/ml, 6 μg/ml, 8 μg/ml, 10 μg/ml, 12 μg/ml, 14 μg/ml, 16 μg/ml, 18 μg/ml, 20 μg/ml |
| Incubation Time: | 24-72 hours |
| Result: | Inhibited HepG2 cells survival. |
Apoptosis Analysis
| Cell Line: | HepG2 cells |
| Concentration: | 48 hours |
| Incubation Time: | 10, 20, and 30 μg/ml |
| Result: | Induced the proportion of annexin V-stained cells in both the early and late apoptotic stage. |
Western Blot Analysis
| Cell Line: | HepG2 cells |
| Concentration: | 48 hours |
| Incubation Time: | 10, 20, and 30 μg/ml |
| Result: | Decreased the protein expression of caspase-3, caspases-8 and -9, reduced ICAD and PARP protein expression. |
Leachianone A (intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days) significantly diminishes the tumor volume by 17-54% in LA-treated nude mice, when compared with those solely given the vehicle.
| Animal Model: | Male nude mice with human hepatoma HepG2 cells |
| Dosage: | 20 mg/kg; 30 mg/kg |
| Administration: | Intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days |
| Result: | Suppressed the tumor growth in vivo. |
里查酮 A 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024-11-08 | HY-N2281 | 里查酮 A | 97938-31-3 | 1mg | 900 |
| 2024-11-08 | HY-N2281 | 里查酮 A | 97938-31-3 | 5mg | 2400 |
