硫黄素 T
硫黄素 T 性质
熔点 | 211-212 °C (decomp) |
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密度 | 1.301g/cm3 at 20℃ |
蒸气压 | 0Pa at 25℃ |
储存条件 | Store below +30°C. |
溶解度 | 在甲醇中可溶1mg/mL |
色指数 | 49005 |
形态 | 粉末 |
颜色 | 黄色至黄褐色 |
水溶解性 | water: 5mg/mL |
BRN | 3922452 |
InChI | InChI=1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1 |
InChIKey | JADVWWSKYZXRGX-UHFFFAOYSA-M |
SMILES | C1(=[N+](C)C2C=CC(C)=CC=2S1)C1C=CC(N(C)C)=CC=1.[Cl-] |
LogP | 3.02 at 25℃ and pH7.98 |
CAS 数据库 | 2390-54-7(CAS DataBase Reference) |
EPA化学物质信息 | Benzothiazolium, 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-, chloride (2390-54-7) |
硫黄素 T 用途与合成方法
Thioflavin T (ThT) is a benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils and is commonly used to diagnose amyloid fibrils, both ex vivo and in vitro. In aqueous solutions, Thioflavin T is found to exist as micelles at concentrations commonly used to monitor fibrils by fluorescence assay (~10-20 μM). Specific conductivity changes are measured at varying concentration of Thioflavin T and the critical micellar concentration is calculated to be 4.0±0.5 μM. Changes in the fluorescence excitation and emission of Thioflavin T are also dependent on the micelle formation. The Thioflavin T micelles of 3 nm diameter are directly visualized using atomic force microscopy, and bound Thioflavin T micelles are observed along the fibril length for representative fibrils. Increasing concentration of Thioflavin T above the critical micellar concentration shows increased numbers of micelles bound along the length of the amyloid fibrils. Thioflavin T micelles are disrupted at low pH as observed by atomic force microscopy and fluorescence enhancement upon binding of Thioflavin T to amyloid fibrils also reduced by several-fold upon decreasing the pH to below 3.The micelles of Thioflavin T bind amyloid fibrils leading to enhancement of fluorescence emission.
硫黄素 T 价格(试剂级)
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
---|---|---|---|---|---|
2025-02-05 | 21176 | 硫黄素 T | 2390-54-7 | 5g | 562 |
2025-02-05 | 21176 | 硫黄素 T | 2390-54-7 | 25g | 1245 |