ANTI-HISTONE H3 (PHOSPHO T3)抗体

兔多克隆抗体to Histone H3 (phospho T3) Rabbit 与反应: Human
预测可用于: Mouse, Chimpanzee Unconjugated 适用于: WB

Western blot - Anti-Histone H3 (phospho T3) antibody (ab229614)

All lanes : Anti-Histone H3 (phospho T3) antibody (ab229614) at 1/10000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (treated with 0.1 uM Calyulin for 30 minutes + phosphatase inhibitors) whole cell extract
Lysates/proteins at 2 µg per lane.
Predicted band size: 15 kDa

Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes. 双抗体夹心法测定标本中HISTONE H3(PHOSPHO T3)水平。用纯化的HISTONE H3(PHOSPHO T3)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入HISTONE H3(PHOSPHO T3)，再与HRP标记的HISTONE H3(PHOSPHO T3)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HISTONE H3(PHOSPHO T3)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中HISTONE H3(PHOSPHO T3)浓度。
ANTI-HISTONE H3(PHOSPHO T3)抗体是一类可以特异性结合HISTONE H3(PHOSPHO T3)的多克隆抗体，主要用于检测HISTONE H3(PHOSPHO T3)的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。