5-Azacytosine

• Enzymic synthesis of nucleoside analogs using immobilized 2′-deoxyribosyltransferase from Lactobacillus reuteri
• Reactions with oxide radical ion

• Potential antitumor and antiproliferative agent against human leukemia cells

64-18-6

127099-85-8

931-86-2

(1) Add 1.5Kg of anhydrous formic acid (99.5% purity), 2.5kg of dicyandiamide and 0.005Kg of p-toluenesulfonic acid catalyst to a 10L autoclave. Under the condition of sealing and stirring, the reaction system was slowly warmed up to 110°C and the reaction pressure was maintained. After the reaction was completed, the temperature was slowly cooled down to room temperature to obtain a white solid product. (2) The resulting white solid was transferred to a 50L glass reactor and 10L of dilute hydrochloric acid solution with a mass concentration of 5% was added. Heating was refluxed for 30 minutes until the solution was clarified. Subsequently, the mixture was filtered through a cartridge into a crystallizer. A 30% ammonia solution was added to the crystallizer and the pH was adjusted to 6. White crystals were precipitated. After centrifugation and drying in a hot air oven, 2.8-5 kg of 6-amino-1,3,5-triazin-2(1H)-one (85% yield as dicyandiamide) was finally obtained.