MCA-Pro-Leu-Ala-Gln-Ala-Val-Dap(Dnp)-Arg-Ser-Ser-Ser-Arg-NH2

Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorescent peptide and as one of fluorescent substrates of TNF-α converting enzyme (TACE; ADAM17), ADAM 9 and ADAM 10. Mca-PLAQAV-Dpa-RSSSR-NH2 is a substrate based on fluorescence resonance energy transfer, and its activity can be determined by the change of fluorescence intensity during pyrolysis[1].

ADAM10; ADAM17

[1] Yi Wang, et al. Protease assay method using site-specific fluorescence dye labeled protein as substrate. US9708638. 2017.
[2] Haga S, et al. TACE antagonists blocking ACE2 shedding caused by the spike protein of SARS-CoV are candidate antiviral compounds. Antiviral Res. 2010 Mar;85(3):551-5. DOI:10.1016/j.antiviral.2009.12.001
[3] Inoshima N, et al. Genetic requirement for ADAM10 in severe Staphylococcus aureus skin infection. J Invest Dermatol. 2012 May;132(5):1513-6. DOI:10.1038/jid.2011.462
[4] Liang T, et al. KID24, an antibody directed against ADAM9, is a potent inhibitor of tumor growth in vivo[J]. Molecular Cancer Therapeutics, 2007, 6(11_Supplement): C32-C32.