Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Dimethylation of histone H3 at lysine 9 (H3K9Me2) is associated with transcriptional repression, whereas acetylation of histone H3 at lysine 14 (H3K14Ac) is associated with transcriptional activation.3,4 H3K9Me2/K14Ac levels are increased in mouse differentiated embryonic stem cells (ESCs) compared with undifferentiated ESCs.5 Altered H3K9Me2/K14Ac levels have been found in tumor biopsies from patients with HER2+, triple-negative, luminal A-like, or luminal B-like breast cancer.6 Cayman's Histone H3K9Me2/K14Ac Monoclonal Antibody (RM322) recognizes histone H3 only when modified by both dimethylation at K9 and acetylation at K14. This antibody can be used for multiplex-based assay and Western blot (WB) applications.WARNING This product is not for human or veterinary use.
