Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Trimethylation of lysine 9 and phosphorylation of serine 10 on histone H3 (H3K9Me3/S10Ph) is associated with repression of polycomb-regulated genes in differentiated mesenchymal stem cells (MSCs) and resting B cells but not pluripotent embryonic stem cells (ESCs).3 Cayman's H3K9Me3/S10Ph Monoclonal Antibody (RM162) recognizes histone H3 only when modified by both trimethylation at K9 and phosphorylation at S10. This antibody can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.WARNING This product is not for human or veterinary use.
