Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Trimethylation of histone H3 at lysine 9 (H3K9Me3) occludes DNA binding by transcription factors and is associated with gene repression.3 H3K9Me3 is also a binding target of the heterochromatin protein 1 (HP1) family proteins CBX1, CBX3, and CBX5.4 It is recognized by the plant homeodomain (PHD) fingers of chromodomain helicase DNA binding protein 4 (CHD4), a member of the NuRD deacetylase complex that is upregulated in a variety of cancers and implicated in the DNA damage response.5 Cayman's Histone H3K9Me3 Monoclonal Antibody (RM389) can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.WARNING This product is not for human or veterinary use.
