Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Monomethylation of histone H3 at lysine 4 (H3K4Me1) is found at active and primed enhancer regions of gene promoters and H3K4Me1-containing nucleosomes are more efficiently remodeled by the chromatin-remodeling complex BAF than unmarked nucleosomes.3,4 It is also enriched at CpG sites associated with aging in stem and differentiated cells.5 Cayman's Histone H3K4Me1 Monoclonal Antibody (RM140) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.WARNING This product is not for human or veterinary use.
