METHYL ACETIMIDATE HYDROCHLORIDE

White to yellow powder

Methyl acetimidate hydrochloride has been used in pre-crystallization chemical modification of lysine residues.

Methyl acetimidate hydrochloride is an inhibitor of N-methylation of phosphatidylethanolamine. It also prevents the stimulation of purified cardiac sarcolemmal vesicles Ca²⁺-pump activities.

Crystallise the imidate from methanol by adding dry ether to a ratio of 1:1 and cooling at 0o. Filter off the crystals in a cold room, wash them with methanol/ether (1:2), then dry in a vacuum. [Hunter & Ludwig J Am Chem Soc 84 3491 1962.] The free base has b 90-91o/765mm, d 0.867, n 1.403. [Hunter & Ludwig Methods Enzymol 25 585 1973, Beilstein 2 IV 181.]