小鼠抗β-Tubulin单克隆抗体

小鼠单克隆抗体to beta III Tubulin

Mouse

与反应: Mouse, Rat, Cow, Cat, Human, Quail, Common marmoset, Dogfish, Catshark Synthetic peptide corresponding to Rat beta III Tubulin aa 400-500.

适用于: Flow Cyt, ICC/IF, IHC-P, IHC-P, IP, WB

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody- Neuronal Marker (ab78078)

IHC image of ab78078 staining beta III Tubulin in Human cerebellum formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0 epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78078, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset). For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

小鼠抗β-Tubulin单克隆抗体是以β-Tubulin为抗原的免疫蛋白，可以特异性结合β-Tubulin，主要用于检测β-Tubulin的Western blotting、Immunohistocry、ELISA、IF、IHC-P、IHC-F等生物实验。