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NADH (sodium salt hydrate)

Product NameNADH (sodium salt hydrate)
CAS1949720-50-6
MFC21H32N7NaO15P2
MW707.46
EINECS
MOL File1949720-50-6.mol

Chemical Properties

storage temp.	2-8°C
solubility	PBS (pH 7.2): 10 mg/ml
form	A crystalline solid
InChIKey	ICWJKBZZWRQAHY-NQLDJOIXNA-N
SMILES	O[C@@H]1[C@@H]([C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@H](N3C=CCC(C(=O)N)=C3)O2)O)O[C@H]1N1C=NC2=C(N=CN=C12)N)O.[NaH].O \|&1:1,2,3,15,16,17,19,32,r\|

Safety Information

Hazard Codes	Xn
Risk Statements	22
Safety Statements	22-24/25

Usage And Synthesis

Description

NADH is the reduced form of nicotinamide adenine dinucleotide (NAD) that can donate electrons as part of a reducing reaction. In that process, NADH becomes oxidized to produce NAD+. A variety of enzymes use NADH plus H+ to reduce substrates, generating NAD+ as well as the reduced product.

Uses

NADH disodium salt (Disodium NADH) hydrate is an orally active reduced coenzyme. NADH disodium salt hydrate is a donor of ADP-ribose units in ADP-ribosylaton reactions and a precursor of cyclic ADP-ribose. NADH disodium salt hydrate plays a role as a regenerative electron donor in cellular energy metabolism, including glycolysis, β-oxidation and the tricarboxylic acid (TCA) cycle[1].

in vivo

NADH (5 μmol/mouse; i.p.; once) increases urinary excretion of nicotinamide and its metabolites in mice^[2].
NADH (500 mg/kg; i.g.; once) promotes alcohol metabolism and prevents or ameliorates early liver injury caused by acute alcohol exposure in ethanol-loaded mice^[3].
NADH (1000 mg/kg; i.p.; once) enhances tissue NAD⁺ levels in male C57BL/6J mice^[3].

Animal Model:	Male ICR mice^[2]
Dosage:	5 μmol/mouse
Administration:	Intraperitoneal injection or oral administration, once
Result:	Produced significant increases in urinary excretions of nicotinamide (Nam) with intraperitoneal injection. Oral administration did not produce any increases in Nam or its metabolites.

Animal Model:	Male C57BL/6J mice^[3]
Dosage:	500 mg/kg
Administration:	Intragastric administration, 15 min before ethanol administration
Result:	Significantly increased blood acetaldehyde levels in mice administered with alcohol between 30 min and two hours. Significantly reduced the acetaldehyde in the blood after two hours. Inhibited the decrease of NAD⁺/NADH redox ratio in hepatocytes.

References

[1] Ying W. NAD+ and NADH in cellular functions and cell death. Front Biosci. 2006 Sep 1;11:3129-48. DOI:10.2741/2038
[2] Kimura N, et al. Comparison of metabolic fates of nicotinamide, NAD+ and NADH administered orally and intraperitoneally; characterization of oral NADH. J Nutr Sci Vitaminol (Tokyo). 2006 Apr;52(2):142-8. DOI:10.3177/jnsv.52.142
[3] Wu K, et al. NADH and NRH as potential dietary supplements or pharmacological agents for early liver injury caused by acute alcohol exposure. Journal of Functional Foods, 2021, 87: 104852.

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