SB 203580盐酸盐
SB 203580盐酸盐 性质
| 储存条件 | -20C |
|---|---|
| 溶解度 | 不溶于乙醇；不溶于水； DMSO 中≥20.7 mg/mL |
| 形态 | 亮黄色固体 |
| 颜色 | 浅黄至黄色 |
| 水溶解性 | Soluble to 25 mM in water |
| 稳定性 | 吸湿性 |
| InChI | 1S/C21H16FN3OS.ClH/c1-27(26)18-8-4-16(5-9-18)21-24-19(14-2-6-17(22)7-3-14)20(25-21)15-10-12-23-13-11-15;/h2-13H,1H3,(H,24,25);1H |
| InChIKey | WOSGGXINSLMASH-UHFFFAOYSA-N |
| SMILES | Fc1ccc(cc1)c2[nH]c(nc2c4ccncc4)c3ccc(cc3)[S](=O)C.Cl |
SB 203580盐酸盐 用途与合成方法
|
p38 50 nM (IC 50 ) |
p38β2 500 nM (IC 50 ) |
SB 203580 (preincubated with 0-30 μM for 1 h and cultured for 24 h in the presence of 20 ng/mL IL-2) prevents the IL-2-induced proliferation of primary human T cells, murine CT6 T cells, or BAF F7 B cells with an IC
50
of 3-5 μM.
SB203580 blocks PKB phosphorylation (IC
50
3-5 μM). SB203580 inhibitsthe phosphorylation of Ser473 in a dose-dependent manner in both CT6 and activated human T cells and IL-2-responsive BA/F3 F7 B cells.
Cell Proliferation Assay
| Cell Line: | CT6, BA/F3 cell line F7, and PBMC/T cells |
| Concentration: | 0-30 μM |
| Incubation Time: | Preincubated with 0-30 μM SB203580 for 1 h and cultured for 24 h in the presence of 20 ng/mL IL-2 |
| Result: | Prevented the IL-2-induced proliferation of primary human T cells, murine CT6 T cells, or BAF F7 B cells with an IC 50 of 3-5 μM. |
Western Blot Analysis
| Cell Line: | CT6 cells, activated human T cells, and BA/F3 F7 cells |
| Concentration: | 0-30 μM |
| Incubation Time: | Preincubated with 0-30 μM SB203580 for 1 h before stimulating with 20 ng/mL IL-2 for 5 min |
| Result: | Inhibited the phosphorylation of PKB at Ser473 in a dose-dependent manner. |
SB203580 (5 mg/kg/day; intra peritoneal injected daily for 16 consecutive days, in female atymic Nu/Nu mice) treatment, p38WT tumors show a significantly smaller tumor burden when compared with p38TM tumors that were treated in parallel.
| Animal Model: | Six-week-old female atymic Nu/Nu mice CAL27 p38WT and p38TM tumors |
| Dosage: | 5 mg/kg/day |
| Administration: | Intra peritoneal injected daily for 16 consecutive days |
| Result: | After 2 weeks treatment, CAL27 p38WT tumors were significantly smaller; CAL27 p38TM tumors were not affected by the p38 inhibitor (n=10). |
SB 203580盐酸盐 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2026-06-05 | HY-10256AR | SB 203580盐酸盐 | 869185-85-3 | 5 mg | 990 |
| 2026-06-05 | HY-10256AR | SB 203580盐酸盐 | 869185-85-3 | 10 mg | 1485 |
