CONOPHYLLINE
CONOPHYLLINE 用途与合成方法
Conophylline (100 ng/ml; 48 hours) reproduces differentiationinducing activity but not apoptosis-inducing activity of activin.
Conophylline (100 ng/ml; 72 hours) exhibits the differentiation-inducing activity in AR42J cells and converts these cells to endocrine cells.
Conophylline increases the expression of neurogenin-3 by activating p38 mitogen-activated protein kinase to induce differentiation of AR42J cells.
Conophylline reduces the expression of α-SMA and collagen-1 in rat HSC and Lx-2 cells.
Conophylline inhibits DNA synthesis induced by serum.
Conophylline also promots activation of caspase-3 and induces apoptosis in Lx-2 Cells.
Apoptosis Analysis
| Cell Line: | Lx-2 Cells |
| Concentration: | 12 hours |
| Incubation Time: | 0.1 μg/ml |
| Result: | Induced apoptosis |
Western Blot Analysis
| Cell Line: | Lx-2 cells |
| Concentration: | 0.1 μg/ml |
| Incubation Time: | 15 minutes, 30 minutes, 60 minutes, 120 minutes |
| Result: | Increased phospho-JNK. |
Conophylline (0.9 mg/kg; p.o.; daily; for 12 weeks) attenuates formation of the liver fibrosis induced by TAA in vivo.
| Animal Model: | Sprague-Dawley rats (70-80 g) |
| Dosage: | 0.9 mg/kg |
| Administration: | Oral administration; daily; for 12 weeks |
| Result: | Attenuated formation of the liver fibrosis induced by TAA. |
