Dabcyl-K-SARS-CoV-2 Replicase Polyprotein 1ab (3235-3246)-E-EDANS (trifluoroacetate salt)

Dabcyl-K-SARS-CoV-2 replicase polyprotein 1ab (pp1ab) (3235-3246)-E-EDANS is a fluorogenic substrate for severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 main protease (Mpro), also known as 3C-like protease (3CLpro).1,2 Upon enzymatic cleavage by SARS-CoV or SARS-CoV-2 Mpro at the Gln-Ser site, EDANS is separated from the Dabcyl quencher and its fluorescence can be used to quantify SARS-CoV or SARS-CoV-2 Mpro activity.1,2,3 EDANS displays excitation/emission maxima of 340/485 nm, respectively.WARNING This product is not for human or veterinary use.

[1] JOHN T.-A. HSU. Evaluation of metal-conjugated compounds as inhibitors of 3CL protease of SARS-CoV[J]. FEBS Letters, 2004, 574 1-3: 116-120. DOI: 10.1016/j.febslet.2004.08.015
[2] TING XIAO . Effect of dihydromyricetin on SARS-CoV-2 viral replication and pulmonary inflammation and fibrosis[J]. Phytomedicine, 2021, 91: Article 153704. DOI: 10.1016/j.phymed.2021.153704
[3] YUNJEONG KIM. Broad-spectrum antivirals against 3C or 3C-like proteases of picornaviruses, noroviruses, and coronaviruses.[J]. Journal of Virology, 2012, 86 21: 11754-11762. DOI: 10.1128/jvi.01348-12