5-Bromouridine 5'-triphosphate sodium salt

5-Bromouridine 5'-triphosphate (5-BrUTP) is a brominated form of UTP that is used to label RNA during transcription. 5-BrUTP in newly-transcribed RNA is then evaluated immunologically with antibodies. This approach has been used to detect or measure RNA transcription with a variety of imaging and molecular methods.1,2,3,4,5WARNING This product is not for human or veterinary use.

[1] KEN-ICHIRO ABE. Global gene silencing is caused by the dissociation of RNA polymerase II from DNA in mouse oocytes.[J]. Journal of Reproduction and Development, 2010, 56 5: 502-507. DOI: 10.1262/jrd.10-068a
[2] CHRISTOPHER H ESKIW. RNA polymerase II activity is located on the surface of protein-rich transcription factories.[J]. Journal of cell science, 2008, 121 Pt 12: 1999-2007. DOI: 10.1242/jcs.027250
[3] BIANCA S HEINRICH. Protein expression redirects vesicular stomatitis virus RNA synthesis to cytoplasmic inclusions.[J]. PLoS Pathogens, 2010: e1000958. DOI: 10.1371/journal.ppat.1000958
[4] AMJAD JAVED. In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5’-triphosphate labeling.[J]. Methods in molecular biology, 2004, 285: 29-31. DOI: 10.1385/1-59259-822-6:029
[5] M THIRY. Dynamics and three-dimensional localization of ribosomal RNA within the nucleolus.[J]. RNA, 2000, 6 12: 1750-1761. DOI: 10.1017/s1355838200001564