2'(3')-O-Anthraniloylguanosine-5'-O-triphosphate (sodium salt)

2'(3')-O-Anthraniloylguanosine-5'-O-triphosphate (2'-ANT-GTP) and 3'-ANT-GTP are fluorescent GTP derivatives, which undergo spontaneous isomerization.1 2'(3')-ANT-GTP displays an emission maximum of 428 nm upon excitation at 330 nm in water.2 It inhibits mammalian adenylyl cyclase 1 (AC1; Ki = 10 nM in the presence of manganese), B. anthracis AC toxin edema factor (Kis = 4.1 and 13 µM in the presence of manganese and magnesium, respectively), and B. pertussis AC toxin CyaA (Ki = 29 µM in the presence of manganese).1,3,4WARNING This product is not for human or veterinary use.

[1] CIBELE PINTO . Structure–activity relationships for the interactions of 2′- and 3′-(O)-(N-methyl)anthraniloyl-substituted purine and pyrimidine nucleotides with mammalian adenylyl cyclases[J]. Biochemical pharmacology, 2011, 82 4: Pages 358-370. DOI: 10.1016/j.bcp.2011.05.010
[2] HIRATSUKA T. New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as subtrates for various enzymes[J]. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1983, 742 3: Pages 496-508. DOI: 10.1016/0167-4838(83)90267-4
[3] HESHAM M TAHA. Molecular analysis of the interaction of anthrax adenylyl cyclase toxin, edema factor, with 2’(3’)-O-(N-(methyl)anthraniloyl)-substituted purine and pyrimidine nucleotides.[J]. Molecular Pharmacology, 2009, 75 3: 693-703. DOI: 10.1124/mol.108.052340
[4] MARTIN GÖTTLE. Molecular analysis of the interaction of Bordetella pertussis adenylyl cyclase with fluorescent nucleotides.[J]. Molecular Pharmacology, 2007, 72 3: 526-535. DOI: 10.1124/mol.107.034413