ANTI-HUMAN TNF-ALPHA

Monoclonal Mouse

E.coli derived Recombinant Human TNF-α

In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of Recombinant Human TNF-α when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human TNF-α (500-P31ABT) as the detection antibody at a concentration of at least 0.5-1.0 μg/ml.

（PeproTech-500-M26）

To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.

Produced in BALB/c mice immunized with highly pure Recombinant Human TNF-α. Anti-Human TNF- α-specific antibody was purified from cell culture by Protein G affinity chromatography.

ANTI-HUMAN TNF-ALPHA是以ANTI-HUMAN TNF-ALPHA为抗原的免疫蛋白，可以特异性结合HUMAN TNF-ALPHA，主要用于检测HUMAN TNF-ALPHA的Western blotting、Immunohistocry、ELISA、IF、IHC-P、IHC-F等生物实验。