ANTI-HISTONE H3 (PHOSPHO T118)抗体

ELISA - Anti-Histone H3 (phospho T118) antibody (ab33310)
This antibody gave a positive result in ELISA against the immunizing peptide (blue line, ab33505). It gave a negative result in ELISA against the non-modified equivalent peptide (pink line, ab42653). This indicates that it is specific for the modified peptide

背景

Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes.

检测原理

双抗体夹心法测定标本中HISTONE H3(PHOSPHO T118)水平。用纯化的HISTONE H3(PHOSPHO T118)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入HISTONE H3(PHOSPHO T118)，再与HRP标记的HISTONE H3(PHOSPHO T118)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HISTONE H3(PHOSPHO T118)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中HISTONE H3(PHOSPHO T118)浓度。
ANTI-HISTONE H3(PHOSPHO T118)抗体是一类可以特异性结合HISTONE H3(PHOSPHO T118)的多克隆抗体，主要用于检测HISTONE H3(PHOSPHO T118)的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。

组蛋白（histone）

组蛋白（histone）是指所有真核生物的细胞核中，与DNA结合存在的碱性蛋白质的总称。其分子量约10000～20000Kda。
亲缘关系较远的种属中，四种组蛋白（H2A、H2B、H3、H4）氨基酸序列都非常相似，如海胆组织H3的氨基酸序列与来自小牛胸腺的H3的氨基酸序列间只有一个氨基酸的差异，小牛胸腺的H3的氨基酸序列与豌豆的H3也只有4个氨基酸不同。不同生物的H1序列变化较大，在某些组织中，H1被特殊的组蛋白所取代。