Detection of the yolk protein Vtg in plasma from juvenile or male fish is a simple and sensitive biomarker for endocrine disrupting chemicals (EDCs) with estrogenic effects in fish. Quantification of Vtg has become an accepted screening test for the estrogenic effects of EDCs in fish. The Vtg (rainbow trout) ELISA kit is a double-antibody immunometric (sandwich) ELISA for analyzing Vtg in plasma samples. Each kit contains rainbow trout Vtg standard, pre-coated plate(s), blocking/dilution buffer, detecting antibody-AChE conjugate, Ellman’s reagent, PBS-Tween tablets, and complete instructions. A five-plate kit contains 2 vials of Vtg and 2 vials of Ellman’s reagent, sufficient to run two separate assays (for example 2 plates + 3 plates).WARNING This product is not for human or veterinary use.
