Detection of the yolk protein Vtg in plasma from juvenile or male fish is a simple and sensitive biomarker for endocrine disrupting chemicals (EDCs) with estrogenic effects in fish. Quantification of Vtg has become an accepted screening test for the estrogenic effects of EDCs in fish. The Vtg (zebrafish) ELISA kit is a double-antibody immunometric (sandwich) ELISA for analyzing Vtg in whole body homogenates and plasma samples. Each kit contains zebrafish Vtg, pre-coated plate(s), detecting antibody, secondary antibody-HRP conjugate, BSA, OPD-peroxidase substrate, PBS-Tween tablets, PBS tablets, and complete instructions. A five-plate kit contains 2 vials of Vtg and 3 OPD sets, sufficient to run two separate assays (for example 2 plates + 3 plates).
