Detection of the yolk protein Vtg in plasma from juvenile or male fish is a simple and sensitive biomarker for endocrine disrupting chemicals (EDCs) with estrogenic effects in fish. Detection of the yolk protein Vtg in plasma from juvenile or male fish is a simple and sensitive biomarker for endocrine disrupting chemicals (EDCs) with estrogenic effects in fish. Quantification of Vtg has become an accepted screening test for the estrogenic effects of EDCs in fish. The Vtg (medaka) ELISA kit is a double-antibody immunometric (sandwich) ELISA for analyzing Vtg in whole body homogenates samples. Each kit contains detecting antibody, secondary antibody-HRP conjugate, purified medaka Vtg standard, OPD-peroxidase substrate tablets, PBS tablets, PBS-Tween tablets, BSA, pre-coated plate(s), and complete instructions. A five-plate kit contains 2 vials of Vtg and 3 OPD sets, sufficient to run two separate assays (for example 2 plates + 3 plates).WARNING This product is not for human or veterinary use.
