CELLSTAIN- HOECHST 33258 SOLUTION
CELLSTAIN- HOECHST 33258 SOLUTION 性质
| 熔点 | 314 °C |
|---|---|
| 沸点 | 746.9±70.0 °C(Predicted) |
| 密度 | 1.355±0.06 g/cm3(Predicted) |
| 储存条件 | −20°C |
| 溶解度 | 在水中的溶解度10 mg/mL |
| 酸度系数(pKa) | 9.23±0.15(Predicted) |
| 形态 | 粉末 |
| 颜色 | 浅黄至黄色 |
| 稳定性 | 稳定的。与强氧化剂不相容。对光敏感。 |
| EPA化学物质信息 | Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]- (23491-44-3) |
CELLSTAIN- HOECHST 33258 SOLUTION 用途与合成方法
IC50: 51.31±4.56 μM (HeLa cell), 32.43±3.27 μM (HL60 cell), 15.42 ± 2.16 μM (U937 cell)
Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC 50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI 50 from 84×10 -6 to 191.5×10 -6 mol/dm 3 . Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1.
