ChemicalBook Product Catalog Biochemical Engineering Biochemical Reagents Biological Dyes NILE BLUE CHLORIDE

NILE BLUE CHLORIDE

Product NameNILE BLUE CHLORIDE
CAS2381-85-3
MFC20H20N3O.Cl
MW353.85
EINECS219-181-4
MOL File2381-85-3.mol

Chemical Properties

Melting point	>250°C
storage temp.	room temp
solubility	Solubility Soluble in water, ethanol
pka	9.7(at 25℃)
Colour Index	51180
form	Powder
color	Dark blue
PH Range	Blue (9.4) to purple-red (11.0)
Water Solubility	water: 1mg/mL, opaque, blue to very deep blue
ex/em	λex 625, 321 nm; λem 659 nm in MeOH
λmax	638nm
BRN	3923952
Stability	Stable. Incompatible with strong oxidizing agents.
Major Application	CMOS technology, waveguides, electroluminescent device, inks, plastics, biofuel cells, detect bacterial growth, lipids, DNA, diagnosis of diseases related to amyloid accumulation, fluorescent probes, therapy of viral diseases, photodynamic therapy
EPA Substance Registry System	Benzo[a]phenoxazin-7-ium, 5-amino-9-(diethylamino)-, chloride (2381-85-3)

Safety Information

Safety Statements	22-24/25
WGK Germany	3
RTECS	DJ1930000
TSCA	TSCA listed
HS Code	32041990

MSDS

Provider	Language
ACROS	English
SigmaAldrich	English

Usage And Synthesis

Chemical Properties

crystalline solid

Uses

Nile Blue chloride is a histological and lipid stain.

Uses

Nile Blue A 37 is used in microscopy, immunoassays, and in laser technology. It is also suitable for the prevention of deposits during the polymerization of vinyl chloride homo-, co-, and graft polymers.

Preparation

commonly known as the Nile Blue. 5-(Diethylamino)-2-nitrosophenoland Naphthalen-1-amine hydrochloride reaction.

Definition

ChEBI: An organic chloride salt having 5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium as the couterion. fluorescent dye which is also a potent photosensitiser for photodynamic therapy.

Biological Activity

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.

Properties and Applications

bright blue. Soluble in hot water and ethanol are blue. The strong sulfuric acid for orange or brown, after dilute for blu-ray to green and then into a blue. The dye solution to join sodium hydroxide have red precipitation (precipitation soluble in ethyl ether for a green fluorescent light brown orange).

Standard( Cotton )	Light Fastness	Persperation Fastness		Ironing Fastness		Soaping
Standard( Cotton )	Light Fastness	Fading	Stain	Fading	Stain	Fading	Stain
A	1	2-3		3		1

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