Thiocarbamyl nitro blue tetrazolium (TNB) is a chromogenic substrate used in biochemical assays, particularly for detecting enzyme activity. TNB is used as a colorimetric substrate in enzyme assays, where it undergoes a color change upon reduction, allowing for the visual detection of enzymatic activity. TNB is commonly used in research to study enzyme kinetics, screen for enzyme inhibitors, and measure cellular metabolic activity. It is also used in diagnostic tests for identifying enzyme deficiencies or abnormalities.
