(S)-2-Methyl-pyrrolidine

872-32-2

765-38-8

The general procedure for the synthesis of 2-methylpyrrolidine from 2-methylpyrroline was as follows: whole-cell biotransformation reactions were carried out at 30 °C in a shaker at 1000 rpm in a sodium phosphate buffer (100 mM, pH 7.0). Freshly harvested resting cells of E. coli BL21(DE3) expressing IRED with a final OD600nm value of 90 were used.The reaction mixture contained 2.5 or 5 mM of imine substrate and 50 mM of glucose as co-substrate.