双链聚肌胞
双链聚肌胞 性质
| 储存条件 | −20°C |
|---|---|
| 溶解度 | H2O：可溶，10mg/mL |
| 形态 | 冻干粉 |
| 颜色 | 白色至米白色 |
| 水溶解性 | Water : 10 mg/mL (Need ultrasonic and warming) |
双链聚肌胞 用途与合成方法
TLR3, RIG-I, MDA5, apoptosis
Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours.
Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells.
Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1.
Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells.
Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use.
Cell Viability Assay
| Cell Line: | WM793, WM278, WM239A, WM9 and 1205Lu cells |
| Concentration: | 20 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Strongly reduced viability from 100% in controls to 20%–50% within 24 hours. |
Apoptosis Analysis
| Cell Line: | 1205Lu cells |
| Concentration: | 200 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis in 1205Lu cells. |
RT-PCR
| Cell Line: | 1205Lu cells |
| Concentration: | 3 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Induced IFN-β expression in melanoma cells. |
Western Blot Analysis
| Cell Line: | 1205Lu cells |
| Concentration: | 5 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Revealed active subunits of caspase-9 and caspase-8 in melanoma cells. |
Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid.
双链聚肌胞 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024-11-08 | HY-135748 | 5 mg | 560 | ||
| 2024-11-08 | HY-135748 | 双链聚肌胞 | 42424-50-0 | 10mg | 915 |
