生物活性 靶点 体外研究 体内研究 双链聚肌胞 试剂级价格
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双链聚肌胞

双链聚肌胞,42424-50-0,结构式
双链聚肌胞
  • CAS号:42424-50-0
  • 英文名:Polyinosinic-polycytidylic acid sodium salt
  • 中文名:双链聚肌胞
  • CBNumber:CB2187556
  • 分子式:C19H27N7O16P2
  • 分子量:671.41
  • MOL File:42424-50-0.mol
双链聚肌胞化学性质
  • 储存条件 :−20°C
  • 溶解度 :H2O: soluble10mg/mL
  • 形态 :lyophilized powder
  • 颜色 :White to off-white
  • 水溶解性 :Water : 10 mg/mL (Need ultrasonic and warming)
安全信息
  • 安全说明 :22-24/25
  • WGK Germany :3
  • RTECS号 :TR0175000

双链聚肌胞性质、用途与生产工艺

  • 生物活性 Polyinosinic-polycytidylic acid sodium (Poly(I:C) sodium) 是双链 RNA 的合成类似物,是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Polyinosinic-polycytidylic acid sodium 可以用作疫苗佐剂,以增强先天性和适应性免疫反应,并改变肿瘤的微环境,还可以直接触发癌细胞发生凋亡。
  • 靶点

    TLR3, RIG-I, MDA5, apoptosis

  • 体外研究

    Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours.
    Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells.
    Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1.
    Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells.
    Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use.

    Cell Viability Assay

    Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
    Concentration: 20 ng/mL
    Incubation Time: 24 hours
    Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.

    Apoptosis Analysis

    Cell Line: 1205Lu cells
    Concentration: 200 ng/mL
    Incubation Time: 24 hours
    Result: Induced apoptosis in 1205Lu cells.

    RT-PCR

    Cell Line: 1205Lu cells
    Concentration: 3 ng/mL
    Incubation Time: 24 hours
    Result: Induced IFN-β expression in melanoma cells.

    Western Blot Analysis

    Cell Line: 1205Lu cells
    Concentration: 5 ng/mL
    Incubation Time: 24 hours
    Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
  • 体内研究

    Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid.

  • 用途  一种可作为在TLR3水平对细胞信号转导进行研究的模型RNA的双链均聚物。也是一种TRIF依赖性toll样受体-3(TLR3)配体。
双链聚肌胞上下游产品信息
上游原料
下游产品
双链聚肌胞 试剂级价格
  • 更新日期:2024/11/08
  • 产品编号:HY-135748
  • 产品名称:Polyinosinic-polycytidylic acid sodium
  • CAS编号:
  • 包装:5 mg
  • 价格:560元
  • 更新日期:2024/11/08
  • 产品编号:HY-135748
  • 产品名称:双链聚肌胞 Polyinosinic-polycytidylic acid sodium
  • CAS编号:42424-50-0
  • 包装:10mg
  • 价格:915元
双链聚肌胞生产厂家
  • 公司名称:四川库利南科技有限公司
  • 联系电话:400-1166-196 18981987031
  • 电子邮件:cdhxsj@163.com
  • 国家:中国
  • 产品数:11707
  • 优势度:57
  • 公司名称:湖北康恩化学有限公司
  • 联系电话:15337215803 15337215803
  • 电子邮件:3357312443@qq.com
  • 国家:中国
  • 产品数:232
  • 优势度:56
  • 公司名称:EMMX Biotechnology LLC
  • 联系电话:888-539-0666
  • 电子邮件:info@emmx.com
  • 国家:美国
  • 产品数:8447
  • 优势度:60
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