L-抗坏血酸-2-单磷酸酯三环己铵盐
L-抗坏血酸-2-单磷酸酯三环己铵盐 性质
| 沸点 | 682.7±65.0 °C(Predicted) |
|---|---|
| 密度 | 2.01±0.1 g/cm3(Predicted) |
| 酸度系数(pKa) | 1.38±0.10(Predicted) |
| 形态 | 固体 |
| 颜色 | 米白色至浅黄色 |
| InChI | InChI=1S/C6H9O9P/c7-1-2(8)4-3(9)5(6(10)14-4)15-16(11,12)13/h2,4,7-9H,1H2,(H2,11,12,13)/t2-,4+/m0/s1 |
| InChIKey | MIJPAVRNWPDMOR-ZAFYKAAXSA-N |
| SMILES | OC1=C(OP(O)(O)=O)C(=O)O[C@@H]1[C@H](CO)O |
L-抗坏血酸-2-单磷酸酯三环己铵盐 用途与合成方法
IC50: alkaline phosphatase (ALP); collagen formation; runx2A
L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect . L-Ascorbic acid 2-phosphate (50 µM-250 µM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 µM AsA2-P and 10 nM dexamethasone (Dex), and 250 µM AsA2-P and 5 nM Dex.
Cell Proliferation Assay
| Cell Line: | Human corneal endothelial cells (HCECs) |
| Concentration: | 0.1 mM; 0.3 mM; 1.5 mM |
| Incubation Time: | 2 to 3 weeks with medium exchange every 2 to 3 days |
| Result: | Stimulated HCEC cells growth. |
