兔单克隆抗体[EP459Y] to CD20
Rabbit
Monoclonal Rabbit IgG Clone #001
PerCP
This antibody was obtained from a rabbit immunized with purified, Human CD20 Transfected CHO Cells and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.
适用于: Flow Cyt, ICC/IF, WB, IP, IHC-P
Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [EP459Y] (ab78237)
Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) labeling CD20 with purified ab78237 at 1/10 dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 was used as the secondary antibody. Cells were counterstained with ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200. PBS instead of the primary antibody was used as negative control. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Nuclei were stained with DAPI (blue)
CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed on all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.
双抗体夹心法测定标本中CD20水平。用纯化的CD20抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CD20，再与HRP标记的CD20抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CD20呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中CD20浓度。
ANTI-CD20抗体是一类可以特异性结合CD20的单克隆抗体，主要用于检测CD20的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。
CD20表达于除浆细胞（分泌免疫球蛋白的B细胞）外的发育分化各阶段的B细胞的表面，通过调节跨膜钙离子流动直接对B细胞起作用，在B细胞增殖和分化中起重要的调节作用。CD20抗原是一种B细胞分化抗原，仅位于前B细胞和成熟B细胞，它在95%以上的B细胞性淋巴瘤中表达，而在造血干细胞、血浆细胞和其他正常组织中不表达。
CD20可能通CD（白细胞分化抗原）是指血细胞在分化、成熟过程中的细胞表面标记.CD20表达于除浆细胞（分泌免疫球蛋白的B细胞）外的发育分化各阶段的B细胞的表面,过调节跨膜钙离子流动直接对B细胞起作用,在B细胞增殖和分化中起重要的调节作用。
