Nitroso-PSAP is a sensitive and specific chromogenic reagent for iron (Fe2+), which is used for the colorimetric determination of iron in blood after mineralization with periodic acid. TNitroso-PSAP chelates with iron (Fe2+) to produce a green complex (λmax=756 nm, Epsilon = 4.5 x 10 < sup > 4 < / sup > dm < sup > 3 < / sup > mol < sup > 1 < / sup > cm < sup > 1 < / sup >)[1][2].
[1] TAKAYANAGI M, et al. Direct Colorimetric Determination of Iron in Whole-Blood Based on Chelation with 2-Nitroso-5-(N-Propyl-N-Sulfopropylamino) Phenol (Nitroso-PSAP)[J]. Japanese Journal of Clinical Chemistry, 1985, 14(3): 155-160.
[2] Yokota F, et al. Solid phase colorimetry of trace metal ions based on a tristimulus chromaticity diagram. Simultaneous determination of iron (II) and iron (III)[J]. Analytical Communications, 1997, 34(4): 111-112.
