NONYL ACRIDINE ORANGE

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λ_ex: 489 nm, λ_em: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria^[1]. when Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition^[2].