鼠抗人C-MYC抗体

小鼠单克隆抗体 to c-Myc

Mouse 与反应: Human Unconjugated This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD10/Neprilysin/MME (rh CD10/Neprilysin/MME; Catalog#10805-H07H; NP_000893.2; Tyr52-Trp750). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. 适用于: IHC-P, IP

Immunoprecipitation - Anti-c-Myc antibody [8] (ab17355)

Immunoprecipitation analysis of H9 Human ES cells, using ab17355 to immunoprecipitate c-Myc.
Cells were lysed in lysis buffer before being subjected to immunoprecipitation using the primary antibody overnight. Immunocomplexes were recovered with protein-G-sepharose beads. Samples were resolved by SDS-PAGE before transfer to PVDF membrane. The membranes were incubated with the appropriate antibodies and analysed by western blot.

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 1 (CD1), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD1 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD1 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD1 is also associated with other biochemical processes. 双抗体夹心法测定标本中人C-MYC水平。用纯化的人C-MYC抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入人C-MYC，再与HRP标记的人C-MYC抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的人C-MYC呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中人C-MYC浓度。
鼠抗人C-MYC抗体是一类可以特异性结合人C-MYC的多克隆抗体，主要用于检测人C-MYC的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。

c-myc基因定位于染色体8q24、IgH、IgK、Igλ链的基因位点分别在14q32、2P13和22q11，在BL细胞中往往出现C-myc基因位点与Ig基因位点之间的易位，即C-myc易位到Ig位点的高活性转录区，从而组成一个高转活性的重排基因，启动C-myc转录，使C-myc表达增强，促进细胞恶变，最后导致肿瘤的发生。
一般认为c-myc仅仅是促进细胞增殖的，但也发现它具有双重作用。长期培养的成纤维细胞中加入生长因子，诱导c-myc基因表达，可刺激细胞增殖；当撤去生长因子，且下调c-myc基因表达时，细胞停滞在G1期而不增殖；当撤去生长因子，而上调c-myc时，可见细胞从G1期进入S期，迅速凋亡。即：c-myc和生长因子均阴性时，细胞停滞生长；二者均阳性时，细胞群体增殖；c-myc阳性而生长因子阴性时，细胞进入凋亡。