LY 294002, 4′-NH2 is a cell permeable 4′-amino derivative which inhibits the p110α, p110β, p110δ, and p110γ subunits of PI 3-kinase. LY 294002, 4′-NH2 functions by preventing the phosphorylation of Akt1 in the PI 3-kinase/Akt1/mTOR pathway. In addition, the 4′ end of LY 294002, 4′-NH2 can be immobilized on an epoxy containing solid surface, which is beneficial for binding studies. Moreover, studies suggest that LY 294002, 4′-NH2 interacts with non-PI 3-kinase cellular proteins such as mTOR, ALDH1, Brd4, GSK3β and CKIIα. Another useful characteristic of LY 294002, 4′-NH2 is that it functions as a reversible inhibitor of PI 3-kinase.
