Elastase

한글명:Elastase

카스 번호39445-21-1

상품명:Elastase

CBNumberCB8394260

분자식NULL

포뮬러 무게0

MOL 파일Mol file

화학적 성질

RTECS 번호	TS5052000
저장 조건	-20°C
용해도	H₂O: 용해성
물리적 상태	동결건조된 분말
색상	하얀색
수용성	물에 용해됩니다.

위험 및 안전 성명

위험품 표기	B,Xn
위험 카페고리 넘버	36/37/38-42
안전지침서	23-24-26-36/37-22
WGK 독일	3
F 고인화성물질	3-10

그림문자(GHS)

Elastase 화학적 특성, 용도, 생산

용도
Elastase is used in combination with other proteases for protein analysis by mass spectrometry. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. It is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.
일반 설명
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Biochem/physiol Actions
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.

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