プロテイナーゼK

危険有害性情報のコード(GHS)

• 絵表示(GHS)

  

• 注意喚起語

  Danger

• 危険有害性情報

  H315：皮膚刺激

  H319：強い眼刺激

  H334：吸入するとアレルギー、喘息または、呼吸困難 を起こすおそれ

• 注意書き

  P261：粉じん/煙/ガス/ミスト/蒸気/スプレーの吸入を避ける こと。

  P264：取扱い後は皮膚をよく洗うこと。

  P264：取扱い後は手や顔をよく洗うこと。

  P280：保護手袋/保護衣/保護眼鏡/保護面を着用するこ と。

  P284：呼吸用保護具を着用すること。

  P302+P352：皮膚に付着した場合：多量の水と石鹸で洗うこと。

  P305+P351+P338：眼に入った場合：水で数分間注意深く洗うこと。次にコ ンタクトレンズを着用していて容易に外せる場合は外す こと。その後も洗浄を続けること。

プロテイナーゼK 価格

MSDS Information

• Prok

プロテイナーゼK 化学特性,用途語,生産方法

• 外観

  無色〜わずかにうすい黄色, 澄明の液体

• 溶解性

  水に溶ける。

• 用途

  DNA及びRNAの精製。

• 特長

  疎水性脂質族および芳香族アミノ酸のカルボキシル基を含むペプチド結合を主に分解する。多くのDNA、RNA分解酵素を急速に分解し不活化する作用があるので、核酸の精製に使用される。本品は、遺伝子工学用の製品であるため核酸の抽出に最適である。

• 説明

  Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, though in the absence of this divalent cation some catalytic activity is retained. Maximum proteinase K activity is observed with the inclusion of 1 mM Ca++ in the reaction buffer. Proteinase K digestion is routinely performed at 50°C, and occasionally in the presence of EDTA to inhibit labile, Mg++-dependent nucleases. Proteinase K is prepared commonly as a 20 mg/mL stock solution in sterile water (stable for 1 year at ?20°C) or in a solution of 50 mM Tris, pH 8.0, 1 mM CaCl2 (stable for months at 4°C). It is generally used at a working concentration of up to 50 μg/mL in any of a number of buffer formulations, including those that contain as much as 0.5% SDS[1].
  

• 化学的特性

  Powder beige

• 使用

  A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.

• 定義

  Proteinase K cleaves peptide bonds next to the carboxyl-terminal of aromatic amino acids, hydrophobic amino acids and sulfuric amino acids, within the polypeptide chain. Hence Proteinase K can act as an endoprotease on a wide range of protein substrates. Because of its proteolytic preferences, it is a member of the subtilisin group of serine proteases. The K in Proteinase K refers to its ability to digest Keratin protein found in hairs, nails and hooves. Proteinase K is an essential reagent in molecular biology labs because it is useful in isolating DNA and RNA molecules by degrading and inactivating proteins. Fungus Tritirachium album Limber naturally produces proteinase K.

• 一般的な説明

  Proteinase K, an extracellular endopeptidase is synthesized by the mold, Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein and is characterized with an unhydrolyzed protein chain and autolyzed polypeptide chains.

• 作用機序

  Proteinase K is a serine protease that cleaves peptide bonds in proteins. Its mechanism of action involves several steps:
  Binding: Proteinase K first binds to the protein or nucleic acid substrate through non-specific hydrophobic interactions.
  Activation: Once bound, the enzyme undergoes an activation step where a catalytic serine residue is activated by a histidine residue and a water molecule. This leads to the forming of an active site that can cleave peptide bonds.
  Cleavage: The active site of proteinase K cleaves the peptide bond on the carboxylic acid side of the amino acid residues that contain aromatic, aliphatic or hydrophobic amino acid residues. The enzyme can also cleave peptide bonds on the amide side of glycine residues.
  Product release: After cleavage, the products of the reaction (i.e., peptides) are released from the enzyme.
  The mechanism of action of proteinase K is similar to other serine proteases. Still, it is notable for its ability to function under harsh conditions, such as high temperatures and in the presence of detergents.

プロテイナーゼK 生産企業

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