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Phenylmethylsulfonyl fluoride

CAS No.329-98-6
Chemical Name:Phenylmethylsulfonyl fluoride
CBNumber:CB9454820
Molecular Formula:C7H7FO2S
Formula Weight:174.19
MOL File:329-98-6.mol

Phenylmethylsulfonyl fluoride Property

Melting point 92-95 °C
Boiling point 112 °C16 mm Hg(lit.)
Density 0.797 g/mL at 20 °C
Flash point 222 °F
storage temp. 2-8°C
solubility dry solvents (ethanol, methanol, and 2-propanol): 200 mM Stock solution are stable for months at 4°C.
pka 7.0
form Needles (May Agglomerate)
color White
biological source synthetic
Water Solubility hydrolysis
Sensitive Moisture Sensitive
Merck 14,7542
BRN 2088311
Stability Moisture Sensitive
InChIKey YBYRMVIVWMBXKQ-UHFFFAOYSA-N
CAS DataBase Reference 329-98-6(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII 57KD15003I
EPA Substance Registry System Benzenemethanesulfonyl fluoride (329-98-6)
UNSPSC Code 12352202
NACRES NA.77

Safety

Hazard Codes :F,T,C
Risk Statements :11-34-25-23/24/25
Safety Statements :7-16-45-36/37/39-28A-26-27
RIDADR :UN 3261 8/PG 2
WGK Germany :3
RTECS :XT8050000
F :3-10-21
Hazard Note :Highly Toxic/Corrosive
TSCA :T
HazardClass :8
PackingGroup :III
HS Code :29241990
Toxicity :LD50 (24 hr) in mice (mg/kg): 215 ±55 i.p. (Pinsky)

NFPA 704:

	1
3		1

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordDanger
Hazard statements H301-H314
Precautionary statements P260-P270-P280-P303+P361+P353-P304+P340+P310-P305+P351+P338

Phenylmethylsulfonyl fluoride Price More Price(45)

Brand: Sigma-Aldrich(India)
Product number: P7626
Product name : Phenylmethanesulfonyl fluoride
Purity: ≥98.5% (GC)
Packaging: 250MG
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Brand: Sigma-Aldrich(India)
Product number: P7626
Product name : Phenylmethanesulfonyl fluoride
Purity: ≥98.5% (GC)
Packaging: 1G
Price: ₹2479.25
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: P7626
Product name : Phenylmethanesulfonyl fluoride
Purity: ≥98.5% (GC)
Packaging: 5G
Price: ₹6572.65
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: P7626
Product name : Phenylmethanesulfonyl fluoride
Purity: ≥98.5% (GC)
Packaging: 25G
Price: ₹26216.75
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: P7626
Product name : Phenylmethanesulfonyl fluoride
Purity: ≥98.5% (GC)
Packaging: 100G
Price: ₹87818.2
Updated: 2022/06/14
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Phenylmethylsulfonyl fluoride Chemical Properties,Usage,Production

Chemical Properties White to cream solid
Uses PMSF is an irreversible serine/cysteine protease inhibitor
Uses Phenylmethylsulfonyl fluoride is used as a Protease inhibitor such as Chymotrypsin, Trypsin and Thrombin as well as Acetylcholineesterase.
Uses Standard protease inhibitor in biological research; in protein purification to prevent proteolytic degradation.
Definition ChEBI: Phenylmethanesulfonyl fluoride is an acyl fluoride with phenylmethanesulfonyl as the acyl group. It has a role as a serine proteinase inhibitor. It derives from a phenylmethanesulfonic acid.
General Description In biochemistry, Phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor commonly used in preparation of cell lysates. It is rapidly degraded in water and stock solutions are usually made up in anhydrous ethanol, isopropanol, corn oil, or DMSO (Dimethyl sulfoxide).
Hazard Extremely corrosive and destructive to tissues. May cause irreversible eye damage. PMSF hydrolyzes upon exposure to water/moisture, liberating a toxic and corrosive gas (hydrogen flouride) that in contact with metal surfaces can generate flammable and/or explosive hydrogen gas.
Biological Activity pmsf (phenylmethanesulfonyl fluoride) is an irreversible inhibitor of serine proteinases, which is associated with the development of the delayed organophosphorus neuropathy. it has a role in a lot of cellular repair and regeneration processes in many kinds of tissues. pmsf is a long acting neuropathy target esterase (nte) inhibitor. nte is a protection was related to inhibition of the putative target of organophosphate-induced delayed polyneuropathy (opidp). pmsf can increase nte inhibition to more than 90%. pmsf also acts as an active site directed reagent for γ-glutamyl transpeptidase.thomas baker, herbert e. lowndes, martin k. johnson, irene c. sandborg. the effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. archives of toxicology. 1980; 46(3-4): 305 – 311.marcello lotti, stefano caroldi, eugenio capodicasa, angelo moretto. promotion of organophosphate-induced delayed polyneuropathy by phenylmethanesulfonyl fluoride. toxicology and applied pharmacology. 1991; 108(2): 234 – 241.masayasu inoue, seikoh horiuchi, yoshimasa morino. inactivation of γ-glutamyl transpeptidase by phenylmethanesulfonyl fluoride, a specific inactivator of serine enzymes. biochemical and biophysical research communications. 1978; 82(4): 1183 – 1188.
Biochem/physiol Actions Phenylmethanesulfonyl fluoride has the ability to enhance the stability of plasma lipidome in lipidomic and metabolomic analysis. This serine protease inhibitor reduces the naloxone-precipitated withdrawal jumping behavior in morphine-dependent mice.
storage Desiccate at RT
storage Phenylmethylsulfonyl fluoride (PMSF) is incompatible with water, acids, bases, and strong oxidizing agents. Do not store PMSF in glass or mild steel/galvanized containers. Store in a cool, dry, and well-ventilated area away from incompatible chemicals.
Purification Methods Purify PMSF by recrystallisation from *C6H6, pet ether or CHCl3/pet ether. [Davies & Dick J Chem Soc 483 1932, cf Tullock & Coffman J Org Chem 23 2016 1960.] It is a general protease inhibitor (specific for trypsin and chymotrypsin) and is a good substitute for diisopropylphosphoro floridate [Fahrney & Gould J Am Chem Soc 85 997 1963]. [Beilstein 11 III 331.]