ChemicalBook > CAS DataBase List > HEPES

HEPES

CAS No.7365-45-9
Chemical Name:HEPES
CBNumber:CB5408557
Molecular Formula:C8H18N2O4S
Formula Weight:238.3
MOL File:7365-45-9.mol

HEPES Property

Melting point 234-238 °C
Boiling point 408℃[at 101 325 Pa]
bulk density 560kg/m3
Density 1.07 g/mL at 20 °C
vapor pressure 0Pa at 25℃
refractive index n20/D 1.339
storage temp. 2-8°C
solubility H₂O: 1 M at 20 °C, clear, colorless
form powder
color White
PH 5.0-6.5 (1Maqueous solution)
pka 7.5(at 25℃)
PH Range 7.0 - 7.6
biological source synthetic
Water Solubility Soluble
λmax λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck 14,4654
BRN 883043
Stability Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP -3.85 at 20℃
CAS DataBase Reference 7365-45-9(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII RWW266YE9I
EPA Substance Registry System 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)
Absorption ≤0.05 at 290 in H2O at 33%
UNSPSC Code 41116107
NACRES NA.75

Safety

Hazard Codes :Xi
Risk Statements :36/37/38
Safety Statements :24/25-22-36-26
WGK Germany :1
RTECS :TL6809000
TSCA :Yes
HS Code :29335995

NFPA 704:

	1
2		1

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordWarning
Hazard statements H302-H315-H319-H335
Precautionary statements P261-P271-P280

HEPES Price More Price(60)

Brand: Sigma-Aldrich(India)
Product number: RDD002
Product name : HEPES
Purity: anhydrous, free-flowing, Redi-Dri?, ≥99.5%
Packaging: 1KG
Price: ₹65361.35
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: RDD002
Product name : HEPES
Purity: anhydrous, free-flowing, Redi-Dri?, ≥99.5%
Packaging: 2.5KG
Price: ₹107427.3
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: H7523
Product name : HEPES
Purity: BioXtra, pH 5.0-6.5 (1?M in H2O), ≥99.5% (titration)
Packaging: 50G
Price: ₹11582.75
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: PHR1428
Product name : HEPES
Purity: Pharmaceutical Secondary Standard; Certified Reference Material
Packaging: 1G
Price: ₹8346.08
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: H6147
Product name : HEPES
Purity: BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
Packaging: 25G
Price: ₹6765.63
Updated: 2022/06/14
Buy: Buy

HEPES Chemical Properties,Usage,Production

Description HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chemical Properties White crystalline powder
Uses Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
Uses HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
Uses HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay.
Definition ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
General Description HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Biological Activity Multi purpose buffer used in biological research.
Biological Activity HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
storage Room temperature
Purification Methods Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]