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Thrombin

CAS No.9002-04-4
Chemical Name:Thrombin
CBNumber:CB3387359
Molecular Formula:NULL
Formula Weight:0
MOL File:Mol file

Thrombin Property

Melting point 47.7 °C
storage temp. -20°C
solubility H₂O: 1 mL/vial clear to slightly hazy, colorless solution
form powder
color white
biological source rabbit plasma
Water Solubility water: soluble
Merck 13,9461
Specific Activity 80-300NIH units/mg protein
EWG's Food Scores 1
FDA UNII 25ADE2236L
ATC code B02BC06,B02BD30
EPA Substance Registry System Thrombin (9002-04-4)
UNSPSC Code 12352204
NACRES NA.75

Safety

Hazard Codes :Xn,B,Xi
Risk Statements :36/37/38-42
Safety Statements :22-24/25-36/37-26-24
WGK Germany :2
RTECS :XO8950000
TSCA :Yes
HS Code :30021099

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordDanger
Hazard statements H315-H319-H334-H335
Precautionary statements P261-P264-P271-P302+P352-P304+P340+P312-P305+P351+P338

Thrombin Price More Price(44)

Brand: Sigma-Aldrich(India)
Product number: T8885
Product name : Thrombin from human plasma
Purity: lyophilized powder, Suitable for routine use in the thrombin time test
Packaging: 10VIALS
Price: ₹21300.9
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: T7513
Product name : Thrombin from bovine plasma
Purity: lyophilized powder, ≥2,000?NIH units/mg protein (E1%/280 = 19.5)
Packaging: 50UNITS
Price: ₹11877
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: T8885
Product name : Thrombin from human plasma
Purity: lyophilized powder, Suitable for routine use in the thrombin time test
Packaging: 25VIALS
Price: ₹38983.2
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: T7513
Product name : Thrombin from bovine plasma
Purity: lyophilized powder, ≥2,000?NIH units/mg protein (E1%/280 = 19.5)
Packaging: 100UNITS
Price: ₹17493.6
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: T7513
Product name : Thrombin from bovine plasma
Purity: lyophilized powder, ≥2,000?NIH units/mg protein (E1%/280 = 19.5)
Packaging: 250UNITS
Price: ₹33977.1
Updated: 2022/06/14
Buy: Buy

Thrombin Chemical Properties,Usage,Production

Description In order to stop local bleeding, thrombin, a natural thrombin drug that catalyzes the transformation of fibrinogen to fibrin, is frequently used. Thrombin is synthesized from cow plasma, and it is used to stop bleeding from open vessels when it is not possible to use other methods. It is used to stop light bleeding.
Uses Thrombostat (Parke-Davis).
Uses Thrombin is a trypsin-like serine protease involved in a multitude of processes in the human body. Thrombin generation is the result of limited proteolysis of the vitamin K-dependent zymogen prothrombin. Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to fibrin which forms the fibrin gel of a haemostatic plug or a pathologic thrombus
Uses Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.
General Description Thrombin is a sterile protein substanceprepared from prothrombin of bovine origin. It is used as atopical hemostatic because it can clot blood, plasma, or a solutionof fibrinogen without addition of other substances.Thrombin also may initiate clotting when combined withgelatin sponge or fibrin foam.
For external use it is applied topically to the wound, as asolution containing 100 to 2,000 National Institutes ofHealth (NIH) units/mL in sodium chloride irrigation or sterilewater for injection or as a dry powder.
Biochem/physiol Actions The main function of thrombin is the cleavage of fibrinogen to fibrin, to assist stable clot formation. A wide range of mutations in the prothrombin gene contributes to its deficiency resulting in coagulation disorders like dysprothrombinemia and hypoprothrombinemia. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contributes to the progression of Parkinson′s disease. Altered thrombin levels modulates the coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin. Thrombin accumulation in neurofibrillary tangles of the brain may contribute to the aggregation of tau protein and pathophysiology of Alzheimer disease.
Purification Methods Thrombin is purified by chromatography on a DEAE-cellulose column, while eluting with 0.1M NaCl, pH 7.0, followed by chromatography on Sephadex G-200. The final preparation is free from plasminogen and plasmin. [Yin & Wessler J Biol Chem 243 112 1968.] Thrombin from bovine blood is also purified by chromatography using p-chlorobenzylamino--aminocaproyl agarose, and gel filtration through Sephadex G-25. [Thompson & Davie Biochim Biophys Acta 250 210 1971.] Thrombin from various species was purified initially by precipitation of impurities with rivanol. [Miller Nature 184 450 1959.] Tissue inhibitor of metalloproteins (TIMP, from human blood plasma), Mr ~30,000. These are purified by an [anti-human amniotic fluid-TIMP]-Sepharose immuno-affinity column and eluted with 50mM glycine/HCl pH 3.0 buffer that is 0.5M in NaCl, and followed by gel filtration [Cawston et al. Biochem J 238 677 1986].