Description
Acetyl-CoA (75520-41-1) is an essential cofactor for cellular metabolism, being an ‘activated acetate’ that shuttles its moieties back and forth during anabolic, catabolic, and metabolic processes involving fundamental molecules: fatty acids, amino acids, glucose, and ATP.1 In the laboratory, it can be used as a substrate in chloramphenicol acetyltransferase (CAT) transcription reporter assays2 or in histone acetyltransferase (HAT) assays in epigenetics research3.
Chemical Properties
White amorphous powder
Uses
Acetyl CoenzymeA Lithium Salt is an important molecule in enzymatic acetyl transfer reactions. It is the source of the phosphopantetheine group that is added as a prosthetic group to acyl carrier proteins and formyltetrahydrofolate dehydrogenase.
in vivo
Acetyl coenzyme A trilithium blunts pressure overload-induced cardiomyopathy in a mice cardiac pressure overload model by Suppressing maladaptive autophagy[2][3].
Mice deprived of food (but with access to water ad libitum) for 24 h exhibit a significant reduction in total Acetyl coenzyme A levels in several organs, including the heart and muscles, corresponding to a decrease in protein acetylation levels. However, the same experimental conditions have no major effects on Acetyl coenzyme A concentrations in the brain and actually increase hepatic Acetyl coenzyme A and protein acetylation levels[4].
References
[1] WOLFE A J. The acetate switch.[J]. Microbiology and molecular biology reviews : MMBR, 2005: 12-50. DOI:
10.1128/mmbr.69.1.12-50.2005[2] C M GORMAN B H H L F Moffat. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.[J]. Molecular and Cellular Biology, 1982, 2 9: 1044-1051. DOI:
10.1128/mcb.2.9.1044-1051.1982[3] YOUNGJOO KIM John M D Kirk G Tanner. A Continuous, Nonradioactive Assay for Histone Acetyltransferases[J]. Analytical biochemistry, 2000, 280 2: Pages 308-314. DOI:
10.1006/abio.2000.4546
