Uses
Digoxigenin-3-
O-methylcarbonyl-ε-aminocaproic acid-
N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).
Uses
Digoxigenin NHS-ester is an activated ester which reacts with amino groups under mild conditions, attaching the digoxigenin moiety to proteins or 5′-amino substituted oligonucleotides Digoxigenin labeling is used to immuno-tag molecules for detection using anti-digoxigenin antibodies with high affinities and specificity.
General Description
Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH
2-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.
Biochem/physiol Actions
Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ?hybridization.
