ADENOSINE 5'-O-THIOMONOPHOSPHATE DILITHIUM SALT

Purification Methods

Purify it as for the diNa salt [Murray & Atkinson Biochemistry 7 4023 1968]. Dissolve 0.3g in dry MeOH (7mL) and 1M LiI (6mL) in dry Me2CO containing 1% of mercaptoethanol, and the Li salt is precipitated by adding Me2CO (75mL). The residue is washed with Me2CO (4 x 30mL) and dried at 55o/25mm. UV: max (HCl, pH 1.2) 257nm ( 14,800); (0.015M NaOAc, pH 4.8) 259nm ( 14,800); and (0.015 4OH, pH 10.1) 259nm ( 15,300).