Chemical Properties
Light yellow powder
Uses
Dansylhydrazine can exhibit fluorescence, which can be used for the following:
- monitoring air pollution by detection of the hydrazones formed by acid-catalyzed derivatization of the carbonyl compounds
- detection of the separated glycoproteins via sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)
Uses
Dansylhydrazine (DH) is widely used as a fluorometric reagent for carbonyl compounds. Dansylated carbonyl molecules can be separated and identified by analytical techniques.
General Description
Dansylhydrazine (DNSH) is a fluorescent reagent which is commonly utilized for derivatization of carbonyl compounds.
Synthesis
Using 4'-aminoazobenzene-4-sulfonic acid as raw material, N,N-dimethylaminoazobenzene-4-sulfonic acid was made by methylation; then 4-dimethylaminophenyl azobenzenesulfonyl chloride was made by chlorination with phosphorus pentachloride; 4-dimethylaminophenyl azobenzenesulfonyl chloride was amidated with hydrazine to obtain dansulfonyl hydrazine.
