Description
7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that is commonly used for the detection or exclusion of non-viable cells in flow cytometric analysis, as it is generally excluded by live cells. It displays excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm. As 7-AAD is detected in the far red range of the spectrum, it exhibits minimal spectral overlap with commonly used probes, therefore it can be used in conjunction with probes such as FITC. 7-AAD has been used to evaluate apoptosis and cell-mediated cytotoxicity and to stain DNA in cells that have been fixed and permeabilized by a variety of methods.
Chemical Properties
Purple powder
Uses
7-Aminoactinomycin D is a fluorescent DNA dye for apoptosis and flow cytomtery studies.
Uses
Fluorescent DNA stain. A featured product for Apoptosis research.
Definition
ChEBI: 7-aminoactinomycin D is a chromopeptide. It has a role as a fluorochrome.
General Description
Free form: Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nm
Emission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nm
DNA Complex: Excitation: 543 nm; 555 nm
Emission: 655 nm; 665 nm
Biochem/physiol Actions
Primary Targetleukemias and sarcomas
References
[1] TANJA TELIEPS. Cellular-FLIP, Raji isoform (c-FLIPR) modulates cell death induction upon T-cell activation and infection[J]. European Journal of Immunology, 2013, 43 6: 1499-1510. DOI:
10.1002/eji.201242819[2] INGRID SCHMID Beth D J Christel Uittenbogaart. Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D[J]. Nature Protocols, 2007, 2 1: 187-190. DOI:
10.1038/nprot.2006.458[3] K TOBA. Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY.[J]. European Journal of Haematology, 2000, 64 1: 10-21. DOI:
10.1034/j.1600-0609.2000.09005.x[4] HERVÉ LECOEUR. Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods[J]. Journal of immunological methods, 1997, 209 2: Pages 111-123. DOI:
10.1016/s0022-1759(97)00138-5[5] X SU. Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect.[J]. Journal of immunology, 1996, 156 11: 4198-4208.
Abs/Em Maxima
527 (complex)/640 (complex) nm
